plant tissue calture

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Plant tissue culture lighting

In biological research, plant tissue culture refers to a method in which parts of a tissue are introduced into a new, artificial environment, where they continue to function or grow. While parts of a tissue are often used, it is important to note that entire organs are also used for tissue culture goals. Hence, such growth media as agar are used to facilitate this process.


What is light?  Light is energy, and white light contains all the colors and wavelength of light. The photosynthesis process allows plant to capture the light energy and turn in to chemical energy in the form of carbohydrate.

The quality and intensity of incident light on culture may play an effective role in the pro­motion of organogenesis. Studies on spectral light on organogenesis reveals that the blue re­gion of the spectrum promotes shoot formation and red light induces rooting.

Type of light source will affect light intensity in plant tissue culture. Various types of light source can be used in tissue culture including incandescent bulb, tubular fluorescent, metal halide and high-pressure sodium lamps. Incandescent bulb is popular in photoperiodic. Besides these two types, others can be used for photosynthesis. Tubular fluorescent lamps (TFLs) are principally used in multiple-layer tissue cultures (TC) and transplants production .HF electronic fluorescent lighting system with light regulation provides not only energy saving but also the capability of adapting the quantity of light to the stages in the development of the tissue culture plants. 

Tubular fluorescent lamps (TFLs) are principally used in multiple-layer tissue cultures (TC) and transplants production. HF electronic fluorescent lighting system with light regulation provides not only energy saving but also the capability of adapting the quantity of light to the stages in the development of the TC plants. Direction, uniformity, quality of light and various efficient light sources were studied worldwide. LEDs have been proposed as a primary light source for space-based plant research chamber or bio regenerative life support systems.

Led light plant tissue culture

 Super bright red, blue and far-red light-emitting diodes (LEDs) have many advantages over conventional light source for photosynthesis and morphology research. Simulation models of spatial distribution of intensity, conversion factors among photometric, radiometric, and quantum units and the electrical energy efficiency of TFLs and LEDs were discussed. The cost effectiveness of using LEDs in commercial TC and transplants production was discussed. A growth chamber using LEDs as light source, capable of adjusting light quantity and quality, can be a great tool for research and the development of such a system can be cost effective.

Importance of light in tissue culture

Plant tissue culture in agriculture developed a biotechnology of the twentieth century, after decades of development, the technology based on theory and practical applications have gained fast development. Early reports of tissue culture, focused on the study of various types of multi-media composition of plants, including the kind and concentration ratio of inorganic salts, organic compounds, hormones and other media components. With the accumulation of more and more in recent years, media studies, people's research gradually penetrated into flower cultivation conditions, such as incubation temperature, light intensity, the air inside the container factors on tissue culture and plant growth rate during the cultivation, growth quality. 

one of the important conditions of Tissue culture is illumination, mainly in light intensity, light quality, and illumination time aspects: 
1, the light intensity (light intensity) 
Light intensity has an important impact on the proliferation and differentiation of cultured cells, organs, from the current study, the light intensity of foreign plants, initially dividing cells have a significant effect. In general, the light intensity in plant tissue culture is strong, sturdy seedling growth, and the light intensity is weak leggy seedlings easy. 
2, light quality (light wave) 
Light quality on callus induction, training has a significant impact on the proliferation and differentiation of organ tissue. Such as lily bulbils in the red light training, eight weeks later, the differentiation of callus. However, under the blue train, a few weeks after the emergence of callus, and gladiolus ball block inoculation 15 days after sub-cultured buds first appear in blue, seedlings formed strong growth, while the white light seedling slender. According to the study, such as the carnation Ni De Xiang showed the highest growth under white light conditions, followed by red, yellow, green, blue to inhibit the growth, chlorophyll synthesis inhibition monochromatic light, chlorophyll synthesis requires the composite light conditions under complete. 
3, photoperiod (light period) 
To select a certain light-dark cycle of cultured plantlets for tissue culture, the most commonly used light cycle is 16h, 8h darkness. Research has shown that short-day varieties sensitive organs and tissues, in short day easy differentiation, and produce callus under long-day, sometimes dark culture, especially Calli plants under cultivation in the dark than in the light under the better. Such as safflower, Bracteatum callus. 
For more plant tissue culture industry is a necessary need high biological demand for energy efficiency, Jinan Prandtl Biotechnology Co., Ltd. After three years of research and development, according to the conditions under tissue culture, plant photosynthesis and morphogenesis of the spectrum needs through a lot of experiments to ascertain the best ratio of red and blue white tissue culture under the conditions needed for the successful development of a tissue culture-specific LED grow Light tissue culture-specific light. The special light of available tissue culture, tissue culture industry will change the high energy consumption, inefficient embarrassment. 

Prandtl tissue culture research and development of LED Lamp, designed for 11w, lamp life of more than 30,000 hours, with 1,000 square meters of tissue culture room for example, the needs of training aircraft 300 sets, 3000 sets the lamp needs. If GP28 spectrum lamps, lighting 16 hours a day, every day needs electricity 1344 degrees, while 1.5 times the electricity required to cool, about 2000 degrees cooling energy consumption, total 3344 workers total electricity a day. The use of tissue culture dedicated LED lights, power 11w, daily electricity demand for lighting 528w, while 0.5 times the electricity needed to cool down, you will need 264 kWh of electricity a day total 792. This calculation uses LED grow light special tissue culture, tissue culture is not only conducive to a day can save energy 2550 degrees. According to 300 days of production date, annual savings of 765,000 kWh of electricity, the current agricultural electricity 0.6 yuan / kWh calculated can reduce the cost of 460,000 yuan per year. 
Prandtl company developed special LED lights in tissue culture, tissue culture under situation based on plant photosynthesis and morphogenesis of the special requirements 450nml blue 660nm red light district area and developed, LED lamp uses tissue culture-specific red, blue three-color ratio, at 450nanometer and 660nanometer with double peaks, a complete spectrum of specific peak. Light intensity in plant tissue culture affects photosynthesis ability.

Be careful that the range on the lux meter was changed depending on the environment outside and inside to get more exact values of lux


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